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Genechem antisense sequence against pir 19521
Antisense Sequence Against Pir 19521, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antisense+sequences/19521+against+antisense+pir+sequence/pm41109384-72-7-25
Average 86 stars, based on 1 article reviews
antisense sequence against pir 19521 - by Bioz Stars, 2026-11
86/100 stars

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other:

Article Title: Intensive stretch-activated CRT-PMCA1 feedback loop promoted Ca2+ overloading and apoptosis of myoblasts
Article Snippet: Adenovirus vector–mediated CRT and PMCA1 siRNA infection The CRT small interference RNA (siRNA) plasmids (siRNA1: sense sequences 5’- GCAGACCCUGCCAUCUAUUTT-3’; antisense sequences 5’-AAUAGAUGGCAGGG UCUGCTT-3’), (siRNA2: sense sequences 5’-GGACCAGAAGGACAUGCA UTT-3’; antisense sequences 5’- AUGCAUGUCCUUCUGGUCCTT-3’), (siRNA3: sense sequences 5’-GCCCAAGAAGAUUAAGGAUTT-3’; antisense sequences 5’-AUCCUUAAUCUUCUUGGGCTT-3’), and PMCA1 siRNA plasmids (siRNA1: sense sequences 5’-CUUGGGCCAUGCGUUCUAUTT-3’; antisense sequences 5’-AUAGA ACGCAUGGCCCAAGTT-3’), (siRNA2: sense sequences 5’-GGGAUCUUCAACAA CGCCATT-3’; antisense sequences 5’- Page 6/25 UGGCGUUGUUGAAGAUCCCTT-3’), (siRNA3: sense sequences 5’-CGUGGCCAGAUCUUGUGGUTT-3’; antisense sequences 5’-ACCACAAGAUCUGGCCACGTT-3’) were constructed by Genechem Biological Company.

Article Title: Landscape of tumor suppressor long noncoding RNAs in breast cancer
Article Snippet: For the knockdown of EPB41L4A-AS2 expression, two human EPB41L4A-AS2-targeted RNAi sequences (RNAi #1225 sense: TTGATGGAGTTTCCTCTCATA; RNAi #1225 antisense: TATGAGAGGAAACTCCATCAA; RNAi #1237 sense: TTGATGGAGTTTCCTCTCATA; and RNAi# 1237 antisense: TATGAGAGGAAACTCCATCAA) were obtained from GeneChem, Co., Ltd. (Shanghai, China).

Article Title: Analysis of Differentially Expressed Genes in Endothelial Cells Following Tumor Cell Adhesion, and the Role of PRKAA2 and miR-124-3p
Article Snippet: The infection of antisense miR-124-3p sequences (GGCATTCACCGCGTGCCTTA) was done according to Shanghai GeneChem Corporation’s operation manual.

Article Title: MiR-106b inhibitors sensitize TRAIL-induced apoptosis in hepatocellular carcinoma through increase of death receptor 4
Article Snippet: For xenograft, HepG2 cells were transfected with 5 × 10 5 transducing units of empty lentivirus (LT-control) or recombinant lentivirus contained miR-106b antisense nucleotide sequences (LT-anti-miR-106b) (Genechem Co., Ltd).

Incubation:

Article Title: Nuclear translocation of ATG5 induces DNA mismatch repair deficiency (MMR-D)/microsatellite instability (MSI) via interacting with Mis18α in colorectal cancer.
Article Snippet: Department of Pharmacology, School of Basic Medical Sciences, Capital Medical University, Beijing, China Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing, China Department of Experimental Pathology, College of Basic Medical Sciences, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China Department of Pathology, Hospital of Bin Zhou Medical College, Binzhou, China Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, China

Negative Control:

Article Title: Nuclear translocation of ATG5 induces DNA mismatch repair deficiency (MMR-D)/microsatellite instability (MSI) via interacting with Mis18α in colorectal cancer.
Article Snippet: Department of Pharmacology, School of Basic Medical Sciences, Capital Medical University, Beijing, China Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing, China Department of Experimental Pathology, College of Basic Medical Sciences, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China Department of Pathology, Hospital of Bin Zhou Medical College, Binzhou, China Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, China

Synthesized:

Article Title: Nuclear translocation of ATG5 induces DNA mismatch repair deficiency (MMR-D)/microsatellite instability (MSI) via interacting with Mis18α in colorectal cancer.
Article Snippet: Department of Pharmacology, School of Basic Medical Sciences, Capital Medical University, Beijing, China Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing, China Department of Experimental Pathology, College of Basic Medical Sciences, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China Department of Pathology, Hospital of Bin Zhou Medical College, Binzhou, China Toxicology and Sanitary Chemistry, School of Public Health, Capital Medical University, Beijing, China

Plasmid Preparation:

Article Title: Long Noncoding RNAs Control the Modulation of Immune Checkpoint Molecules in Cancer
Article Snippet: HCT116 and HeLa cells were stimulated with 2 mg/mL LPS (#L2630, Sigma-Aldrich) and continued to cultivate with the same culture medium for 3 hours. .. Plasmid, lentivirus production, and infection For the knockdown of HCP5 and MIAT expression, two human HCP5-targeted RNAi sequences (RNAi# sense: TGCTGATGAGTAGGACATTT; RNAi# antisense: AAATGTCCTACTCATCAGCA) and mouse Miat-targeted RNAi sequences (RNAi# sense: AGATATAGTCTGTAATTGTAA; RNAi# antisense: TTACAATTACAGACTATATCT) were obtained from GeneChem, Co., Ltd. .. Long noncoding RNAs control the modulation of immune checkpoint molecules in cancer 1 Shouping Xu 1,# , Qin Wang 1,# , Yujuan Kang 1 , Jiena Liu 1 , Yanling Yin 1 , Lei Liu 1 , Hao Wu 1 , Siwei 2 Li 1 , Shiyao Sui 1 , Meiying Shen 1 , Wei Zheng 1 , Da Pang 1,2* 3 Shouping Xu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 4 China; E-mail: Shoupingxu@hrbmu.edu.cn 5 Qin Wang, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 6 China; E-mail: wangqin0923@163.com 7 Yujuan Kang, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 8 China; E-mail: 991979837@qq.com 9 Jiena Liu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 10 China; E-mail: liujienana@163.com 11 Yanling Yin, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 12 China; E-mail: yinyanling426@163.com 13 Lei Liu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 14 China; E-mail: 13263530798@163.com 15 Hao Wu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 16 China; E-mail: wh849399985@qq.com 17 Siwei Li, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 18 China; E-mail: lisiwei396469776@163.com 19 Shiyao Sui, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 20 China; E-mail: ssy19881227@126.com 21 Meiying Shen, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 22

Article Title: Long Noncoding RNAs Control the Modulation of Immune Checkpoint Molecules in Cancer
Article Snippet: HCT116, HeLa, 4T1, 786-O, Hs578T, BT549, and MCF7 cells obtained from ATCC in 2017 were cultured in RPMI1640 or DMEM and were not authenticated in the past year (Gibco). .. Long noncoding RNAs control the modulation of immune checkpoint molecules in cancer 1 Shouping Xu 1,# , Qin Wang 1,# , Yujuan Kang 1 , Jiena Liu 1 , Yanling Yin 1 , Lei Liu 1 , Hao Wu 1 , Siwei 2 Li 1 , Shiyao Sui 1 , Meiying Shen 1 , Wei Zheng 1 , Da Pang 1,2* 3 Shouping Xu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 4 China; E-mail: Shoupingxu@hrbmu.edu.cn 5 Qin Wang, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 6 China; E-mail: wangqin0923@163.com 7 Yujuan Kang, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 8 China; E-mail: 991979837@qq.com 9 Jiena Liu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 10 China; E-mail: liujienana@163.com 11 Yanling Yin, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 12 China; E-mail: yinyanling426@163.com 13 Lei Liu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 14 China; E-mail: 13263530798@163.com 15 Hao Wu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 16 China; E-mail: wh849399985@qq.com 17 Siwei Li, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 18 China; E-mail: lisiwei396469776@163.com 19 Shiyao Sui, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 20 China; E-mail: ssy19881227@126.com 21 Meiying Shen, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 22 ..

Infection:

Article Title: Long Noncoding RNAs Control the Modulation of Immune Checkpoint Molecules in Cancer
Article Snippet: HCT116 and HeLa cells were stimulated with 2 mg/mL LPS (#L2630, Sigma-Aldrich) and continued to cultivate with the same culture medium for 3 hours. .. Plasmid, lentivirus production, and infection For the knockdown of HCP5 and MIAT expression, two human HCP5-targeted RNAi sequences (RNAi# sense: TGCTGATGAGTAGGACATTT; RNAi# antisense: AAATGTCCTACTCATCAGCA) and mouse Miat-targeted RNAi sequences (RNAi# sense: AGATATAGTCTGTAATTGTAA; RNAi# antisense: TTACAATTACAGACTATATCT) were obtained from GeneChem, Co., Ltd. .. Long noncoding RNAs control the modulation of immune checkpoint molecules in cancer 1 Shouping Xu 1,# , Qin Wang 1,# , Yujuan Kang 1 , Jiena Liu 1 , Yanling Yin 1 , Lei Liu 1 , Hao Wu 1 , Siwei 2 Li 1 , Shiyao Sui 1 , Meiying Shen 1 , Wei Zheng 1 , Da Pang 1,2* 3 Shouping Xu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 4 China; E-mail: Shoupingxu@hrbmu.edu.cn 5 Qin Wang, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 6 China; E-mail: wangqin0923@163.com 7 Yujuan Kang, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 8 China; E-mail: 991979837@qq.com 9 Jiena Liu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 10 China; E-mail: liujienana@163.com 11 Yanling Yin, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 12 China; E-mail: yinyanling426@163.com 13 Lei Liu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 14 China; E-mail: 13263530798@163.com 15 Hao Wu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 16 China; E-mail: wh849399985@qq.com 17 Siwei Li, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 18 China; E-mail: lisiwei396469776@163.com 19 Shiyao Sui, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 20 China; E-mail: ssy19881227@126.com 21 Meiying Shen, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 22

Article Title: Long Noncoding RNAs Control the Modulation of Immune Checkpoint Molecules in Cancer
Article Snippet: HCT116, HeLa, 4T1, 786-O, Hs578T, BT549, and MCF7 cells obtained from ATCC in 2017 were cultured in RPMI1640 or DMEM and were not authenticated in the past year (Gibco). .. Long noncoding RNAs control the modulation of immune checkpoint molecules in cancer 1 Shouping Xu 1,# , Qin Wang 1,# , Yujuan Kang 1 , Jiena Liu 1 , Yanling Yin 1 , Lei Liu 1 , Hao Wu 1 , Siwei 2 Li 1 , Shiyao Sui 1 , Meiying Shen 1 , Wei Zheng 1 , Da Pang 1,2* 3 Shouping Xu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 4 China; E-mail: Shoupingxu@hrbmu.edu.cn 5 Qin Wang, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 6 China; E-mail: wangqin0923@163.com 7 Yujuan Kang, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 8 China; E-mail: 991979837@qq.com 9 Jiena Liu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 10 China; E-mail: liujienana@163.com 11 Yanling Yin, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 12 China; E-mail: yinyanling426@163.com 13 Lei Liu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 14 China; E-mail: 13263530798@163.com 15 Hao Wu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 16 China; E-mail: wh849399985@qq.com 17 Siwei Li, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 18 China; E-mail: lisiwei396469776@163.com 19 Shiyao Sui, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 20 China; E-mail: ssy19881227@126.com 21 Meiying Shen, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 22 ..

Knockdown:

Article Title: Long Noncoding RNAs Control the Modulation of Immune Checkpoint Molecules in Cancer
Article Snippet: HCT116 and HeLa cells were stimulated with 2 mg/mL LPS (#L2630, Sigma-Aldrich) and continued to cultivate with the same culture medium for 3 hours. .. Plasmid, lentivirus production, and infection For the knockdown of HCP5 and MIAT expression, two human HCP5-targeted RNAi sequences (RNAi# sense: TGCTGATGAGTAGGACATTT; RNAi# antisense: AAATGTCCTACTCATCAGCA) and mouse Miat-targeted RNAi sequences (RNAi# sense: AGATATAGTCTGTAATTGTAA; RNAi# antisense: TTACAATTACAGACTATATCT) were obtained from GeneChem, Co., Ltd. .. Long noncoding RNAs control the modulation of immune checkpoint molecules in cancer 1 Shouping Xu 1,# , Qin Wang 1,# , Yujuan Kang 1 , Jiena Liu 1 , Yanling Yin 1 , Lei Liu 1 , Hao Wu 1 , Siwei 2 Li 1 , Shiyao Sui 1 , Meiying Shen 1 , Wei Zheng 1 , Da Pang 1,2* 3 Shouping Xu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 4 China; E-mail: Shoupingxu@hrbmu.edu.cn 5 Qin Wang, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 6 China; E-mail: wangqin0923@163.com 7 Yujuan Kang, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 8 China; E-mail: 991979837@qq.com 9 Jiena Liu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 10 China; E-mail: liujienana@163.com 11 Yanling Yin, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 12 China; E-mail: yinyanling426@163.com 13 Lei Liu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 14 China; E-mail: 13263530798@163.com 15 Hao Wu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 16 China; E-mail: wh849399985@qq.com 17 Siwei Li, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 18 China; E-mail: lisiwei396469776@163.com 19 Shiyao Sui, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 20 China; E-mail: ssy19881227@126.com 21 Meiying Shen, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 22

Article Title: Long Noncoding RNAs Control the Modulation of Immune Checkpoint Molecules in Cancer
Article Snippet: HCT116, HeLa, 4T1, 786-O, Hs578T, BT549, and MCF7 cells obtained from ATCC in 2017 were cultured in RPMI1640 or DMEM and were not authenticated in the past year (Gibco). .. Long noncoding RNAs control the modulation of immune checkpoint molecules in cancer 1 Shouping Xu 1,# , Qin Wang 1,# , Yujuan Kang 1 , Jiena Liu 1 , Yanling Yin 1 , Lei Liu 1 , Hao Wu 1 , Siwei 2 Li 1 , Shiyao Sui 1 , Meiying Shen 1 , Wei Zheng 1 , Da Pang 1,2* 3 Shouping Xu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 4 China; E-mail: Shoupingxu@hrbmu.edu.cn 5 Qin Wang, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 6 China; E-mail: wangqin0923@163.com 7 Yujuan Kang, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 8 China; E-mail: 991979837@qq.com 9 Jiena Liu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 10 China; E-mail: liujienana@163.com 11 Yanling Yin, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 12 China; E-mail: yinyanling426@163.com 13 Lei Liu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 14 China; E-mail: 13263530798@163.com 15 Hao Wu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 16 China; E-mail: wh849399985@qq.com 17 Siwei Li, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 18 China; E-mail: lisiwei396469776@163.com 19 Shiyao Sui, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 20 China; E-mail: ssy19881227@126.com 21 Meiying Shen, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 22 ..

Expressing:

Article Title: Long Noncoding RNAs Control the Modulation of Immune Checkpoint Molecules in Cancer
Article Snippet: HCT116 and HeLa cells were stimulated with 2 mg/mL LPS (#L2630, Sigma-Aldrich) and continued to cultivate with the same culture medium for 3 hours. .. Plasmid, lentivirus production, and infection For the knockdown of HCP5 and MIAT expression, two human HCP5-targeted RNAi sequences (RNAi# sense: TGCTGATGAGTAGGACATTT; RNAi# antisense: AAATGTCCTACTCATCAGCA) and mouse Miat-targeted RNAi sequences (RNAi# sense: AGATATAGTCTGTAATTGTAA; RNAi# antisense: TTACAATTACAGACTATATCT) were obtained from GeneChem, Co., Ltd. .. Long noncoding RNAs control the modulation of immune checkpoint molecules in cancer 1 Shouping Xu 1,# , Qin Wang 1,# , Yujuan Kang 1 , Jiena Liu 1 , Yanling Yin 1 , Lei Liu 1 , Hao Wu 1 , Siwei 2 Li 1 , Shiyao Sui 1 , Meiying Shen 1 , Wei Zheng 1 , Da Pang 1,2* 3 Shouping Xu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 4 China; E-mail: Shoupingxu@hrbmu.edu.cn 5 Qin Wang, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 6 China; E-mail: wangqin0923@163.com 7 Yujuan Kang, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 8 China; E-mail: 991979837@qq.com 9 Jiena Liu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 10 China; E-mail: liujienana@163.com 11 Yanling Yin, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 12 China; E-mail: yinyanling426@163.com 13 Lei Liu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 14 China; E-mail: 13263530798@163.com 15 Hao Wu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 16 China; E-mail: wh849399985@qq.com 17 Siwei Li, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 18 China; E-mail: lisiwei396469776@163.com 19 Shiyao Sui, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 20 China; E-mail: ssy19881227@126.com 21 Meiying Shen, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 22

Article Title: Long Noncoding RNAs Control the Modulation of Immune Checkpoint Molecules in Cancer
Article Snippet: HCT116, HeLa, 4T1, 786-O, Hs578T, BT549, and MCF7 cells obtained from ATCC in 2017 were cultured in RPMI1640 or DMEM and were not authenticated in the past year (Gibco). .. Long noncoding RNAs control the modulation of immune checkpoint molecules in cancer 1 Shouping Xu 1,# , Qin Wang 1,# , Yujuan Kang 1 , Jiena Liu 1 , Yanling Yin 1 , Lei Liu 1 , Hao Wu 1 , Siwei 2 Li 1 , Shiyao Sui 1 , Meiying Shen 1 , Wei Zheng 1 , Da Pang 1,2* 3 Shouping Xu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 4 China; E-mail: Shoupingxu@hrbmu.edu.cn 5 Qin Wang, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 6 China; E-mail: wangqin0923@163.com 7 Yujuan Kang, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 8 China; E-mail: 991979837@qq.com 9 Jiena Liu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 10 China; E-mail: liujienana@163.com 11 Yanling Yin, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 12 China; E-mail: yinyanling426@163.com 13 Lei Liu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 14 China; E-mail: 13263530798@163.com 15 Hao Wu, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 16 China; E-mail: wh849399985@qq.com 17 Siwei Li, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 18 China; E-mail: lisiwei396469776@163.com 19 Shiyao Sui, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 20 China; E-mail: ssy19881227@126.com 21 Meiying Shen, Department of Breast Surgery, Harbin Medical University Cancer Hospital, Harbin, 22 ..



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Ribobio co antisense and sense probes targeting the circ_0046599 sequence
tiRNA-Met directly interacted with SNRPA and promoted its ubiquitination. A , B Silver-stained SDS-PAGE gel images showed proteins immunoprecipitated by tiRNA-Met and its <t>antisense</t> RNA in MDA-MB-231 ( A ) and BT-549 ( B ) cell lines. C Western blot analysis of products from RNA pull-down assays using tiRNA-Met sense and antisense confirmed that tiRNA-Met directly associated with SNRPA. D , E RIP followed by RT-qPCR demonstrated specific binding of tiRNA-Met to SNRPA in MDA-MB-231 ( D ) and BT-549 ( E ) cells. F RNA pull-down and western blot assays revealed that tiRNA-Met bound to the RRM2 domain of SNRPA. G The mRNA expression levels of SNRPA remained unchanged in MDA-MB-231 and BT-549 cells transfected with tiRNA-Met mimics or inhibitor. H Overexpression of tiRNA-Met reduced SNRPA protein levels in both cell lines, while inhibition of tiRNA-Met increased SNRPA protein levels. I MDA-MB-231 cells treated with cycloheximide (Chx; 5 μM) and then transfected with tiRNA-Met mimics exhibited a reduced half-life of endogenous SNRPA protein, as shown by western blot; β-actin served as a loading control. J Treatment with MG132 (20 μg/ml) led to a progressive increase in SNRPA protein levels over time; β-actin was used as a reference. K Immunoprecipitation assays indicated that the binding of SNRPA to ubiquitinated proteins was elevated in MDA-MB-231 cells transfected with tiRNA-Met mimics. All data are presented as means ± SD; ns, P > 0.05; * P < 0.05; ** P < 0.01; **** P < 0.0001
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tiRNA-Met directly interacted with SNRPA and promoted its ubiquitination. A , B Silver-stained SDS-PAGE gel images showed proteins immunoprecipitated by tiRNA-Met and its antisense RNA in MDA-MB-231 ( A ) and BT-549 ( B ) cell lines. C Western blot analysis of products from RNA pull-down assays using tiRNA-Met sense and antisense confirmed that tiRNA-Met directly associated with SNRPA. D , E RIP followed by RT-qPCR demonstrated specific binding of tiRNA-Met to SNRPA in MDA-MB-231 ( D ) and BT-549 ( E ) cells. F RNA pull-down and western blot assays revealed that tiRNA-Met bound to the RRM2 domain of SNRPA. G The mRNA expression levels of SNRPA remained unchanged in MDA-MB-231 and BT-549 cells transfected with tiRNA-Met mimics or inhibitor. H Overexpression of tiRNA-Met reduced SNRPA protein levels in both cell lines, while inhibition of tiRNA-Met increased SNRPA protein levels. I MDA-MB-231 cells treated with cycloheximide (Chx; 5 μM) and then transfected with tiRNA-Met mimics exhibited a reduced half-life of endogenous SNRPA protein, as shown by western blot; β-actin served as a loading control. J Treatment with MG132 (20 μg/ml) led to a progressive increase in SNRPA protein levels over time; β-actin was used as a reference. K Immunoprecipitation assays indicated that the binding of SNRPA to ubiquitinated proteins was elevated in MDA-MB-231 cells transfected with tiRNA-Met mimics. All data are presented as means ± SD; ns, P > 0.05; * P < 0.05; ** P < 0.01; **** P < 0.0001

Journal: Cellular & Molecular Biology Letters

Article Title: The novel tRNA-derived fragment, tiRNA-Met, inhibits the malignant progression of triple-negative breast cancer by regulating RANBP3L via a targeted interaction with SNRPA

doi: 10.1186/s11658-025-00738-2

Figure Lengend Snippet: tiRNA-Met directly interacted with SNRPA and promoted its ubiquitination. A , B Silver-stained SDS-PAGE gel images showed proteins immunoprecipitated by tiRNA-Met and its antisense RNA in MDA-MB-231 ( A ) and BT-549 ( B ) cell lines. C Western blot analysis of products from RNA pull-down assays using tiRNA-Met sense and antisense confirmed that tiRNA-Met directly associated with SNRPA. D , E RIP followed by RT-qPCR demonstrated specific binding of tiRNA-Met to SNRPA in MDA-MB-231 ( D ) and BT-549 ( E ) cells. F RNA pull-down and western blot assays revealed that tiRNA-Met bound to the RRM2 domain of SNRPA. G The mRNA expression levels of SNRPA remained unchanged in MDA-MB-231 and BT-549 cells transfected with tiRNA-Met mimics or inhibitor. H Overexpression of tiRNA-Met reduced SNRPA protein levels in both cell lines, while inhibition of tiRNA-Met increased SNRPA protein levels. I MDA-MB-231 cells treated with cycloheximide (Chx; 5 μM) and then transfected with tiRNA-Met mimics exhibited a reduced half-life of endogenous SNRPA protein, as shown by western blot; β-actin served as a loading control. J Treatment with MG132 (20 μg/ml) led to a progressive increase in SNRPA protein levels over time; β-actin was used as a reference. K Immunoprecipitation assays indicated that the binding of SNRPA to ubiquitinated proteins was elevated in MDA-MB-231 cells transfected with tiRNA-Met mimics. All data are presented as means ± SD; ns, P > 0.05; * P < 0.05; ** P < 0.01; **** P < 0.0001

Article Snippet: Biotin-labeled tiRNA-Met sense and antisense sequences were obtained from Genescript (Nanjing, China).

Techniques: Ubiquitin Proteomics, Staining, SDS Page, Immunoprecipitation, Western Blot, Quantitative RT-PCR, Binding Assay, Expressing, Transfection, Over Expression, Inhibition, Control